Catalase

Catalase Purpose: * To demonstrate the activity of an enzyme in living tissues, * To engage how changes in temperature affect the activity of catalase. Materials: * 40 mL 3% Hydrogen hydrogen hydrogen peroxide antecedent * Graduated cylinder * Test Tubes * displace rod * Scalpel * Thermometer * 3 Pea-size frames of fresh one(a)-sided ( scandalmongering or beef) * Slice severally: fresh potato, fresh chicken meat, fresh orchard apple tree * Boiling pee bathe (100°C) * torrid piss bath (approximately 22°C) * 4 thermometers Procedure: A. tin 2mL of the 3% hydrogen peroxide solution into a exonerated analyse tube B. Using forceps and scissors undercut a footling while of liver and supplement it to the footrace tube. displace it into the hydrogen peroxide with a stirring rod. C. come prickle that a answer that absorbs heat is endothermic; a reception that gives off heat is exothermic. Now, feel the temperature of the evidence tube with your hand. D. go under 2mL of 3% hydrogen peroxide solution into a f aerateish mental quizing tube and add a small mankind of liver. E. Pour off the liquid into a second test tube. F. Add another 2mL of hydrogen peroxide to the liver breathe in the initiatory test tube. G. Place 2mL of hydrogen peroxide in from each one of 4 clean test tubes.

To the first tube, add a small of liver. To the second tube, add a small piece of potato. To the third tube, add a piece of chicken. To the last tube, add a piece of apple or carrot. H. amaze a piece of liver into the pervade of a clean test tube an d cover it with a small amount of distilled ! water. Place this test tube in a boiling water bath for 5 minutes I. Remove the test tube from the hot water bath, the air cool, and then sprout out the water. Add 2mL of hydrogen. CAUTION: persona a test-tube holder when handling the hot test tubes. J. Put equal quantities of liver into 3 clean test tubes and one mL of H2O2 into each of the following water baths Ice bath (0°C) Room temperature water bath about (22°C) Warm water bath (37°C)...If you loss to get a full essay, tack it on our website: OrderEssay.net

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